Journal: Molecular Metabolism

Article Title: AMPK inhibition and elevated angiogenin are associated with tRNA fragmentation in the male germline exposed to a high-fat diet

doi: 10.1016/j.molmet.2026.102350

Figure Lengend Snippet: AMPK inhibition-induced Angiogenin is accompanied by alteration of tRFs profile in vitro. A Western blot results of proteins related with AMPK-mTOR pathways in TM3, TM4, and NIH/3T3 cell lines using compound C (CC), rapamycin (Rapa), and MHY1485 (MHY) in TM3, TM4, and NIH/3T3 cell lines (left to right). The number represents the densiometric comparison of the expression of pAMPK and pmTOR proteins against AMPK and mTOR, respectively. B Angiogenin gene expression. Gene expression was quantified after 12 h of treatment. mRNA expression level was normalized to Actin . Data are represented as mean ± standard deviation. Statistical analysis was performed using One-way ANOVA with Tukey post-hoc test. Different letters are significantly different. C Northern blot results of tRF3-Pro and Val in mouse testis cell lines. Each sample RNAs were extracted from both TM3 and TM4 cells with CC treatment and overexpression of Angiogenin (Ang OE). Each intact tRNA was shown as a control. Band intensities of Northern blot are represented as bar graph after quantification using Image J software. Data are presented as mean ± standard deviation. One-way ANOVA was used for statistical analysis, and the different alphabets with Tukey post-hoc test. D Distribution of read counts based on small RNA type. E Distribution of reads assigned to GtRNAdb in tRF subtypes. The total bar height is proportional to the total read count for each group. Percentages indicate the relative proportion of each tRF subtype within the corresponding group, and n represents the actual read count number for each subtype. tRF5, reads derived from tRNA 5′ end among reads aligned to GtRNAdb; tRF3, reads derived from tRNA 3′ end among reads aligned to GtRNAdb; other, not included in the previous two groups. F Histogram showing the distribution of log 2 (Fold change) using reads aligned to GtRNAdb. Green, CC-treated versus non-treated; Yellow, ANG overexpression versus control. G Volcano plot depicting significantly changed tRFs in TM4 cell lines. Results from CC-treated versus non-treated are represented as circles, and results from ANG overexpression versus control are represented as triangles. n = 2 for each group.

Article Snippet: The mouse testicular TM3 cell line and TM4 Sertoli cell line were purchased from the Korean Cell Line Bank (Seoul, Korea).

Techniques: Inhibition, In Vitro, Western Blot, Comparison, Expressing, Gene Expression, Standard Deviation, Northern Blot, Over Expression, Control, Software, Derivative Assay

Journal: Balkan Medical Journal

Article Title: Fucoxanthin Attenuates Bisphenol A-Induced Testicular Injury via NF-κB-Mediated Pyroptosis Inhibition

doi: 10.4274/balkanmedj.galenos.2026.2025-11-268

Figure Lengend Snippet: Effects of Fx on TM3 cell viability in response to BPA exposure. (a) CCK-8 assay of TM3 cells treated with increasing concentrations of BPA; (b) effects of Fx alone on TM3 cell viability; (c) Fx and C34 attenuate BPA (50 μM)-induced cytotoxicity in TM3 cells. Data presentation and statistical analysis are as described for Figure 1 (n = 6). * p < 0.05, ** p < 0.01, *** p < 0.001. BPA, bisphenol A; Fx, fucoxanthin; DMSO, dimethyl sulfoxide.

Article Snippet: The TM3 Leydig cell line (CL0234), TM3 Cell Complete Medium (CM-0234), and DMEM/F12 (PM150312) were provided by Procell Life Science & Technology (Wuhan, China).

Techniques: CCK-8 Assay

Journal: Balkan Medical Journal

Article Title: Fucoxanthin Attenuates Bisphenol A-Induced Testicular Injury via NF-κB-Mediated Pyroptosis Inhibition

doi: 10.4274/balkanmedj.galenos.2026.2025-11-268

Figure Lengend Snippet: Effects of Fx on the pyroptosis signaling pathway in vitro . (a, b) The mRNA levels of Casp1 and Gsdmd ; (c) Western blotting analysis of the expression of Caspase-1, Caspase-1 p20, GSDMD, and N-GSDMD; (d-f) the quantification of the Western blotting analysis; (g, h) IL-1β and IL-18 concentrations in TM3 cell supernatant. Data presentation and statistical analysis are as described for Figure 1 (n = 3). * p < 0.05, ** p < 0.01, *** p < 0.001. BPA, bisphenol A; Fx, fucoxanthin; GSDMD, gasdermin D; IL-1β, interleukin-1β; IL-18, interleukin-18.

Article Snippet: The TM3 Leydig cell line (CL0234), TM3 Cell Complete Medium (CM-0234), and DMEM/F12 (PM150312) were provided by Procell Life Science & Technology (Wuhan, China).

Techniques: In Vitro, Western Blot, Expressing